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fNIRS explained

What is fNIRS? Learn how light measurements work, what HbO and HbR mean, and how to assess signal quality, interpretation and research outside the laboratory.

Martijn den Otter 5 min read9/29/2026
fNIRS explained

How can you investigate what happens when someone follows instructions, uses an interface or learns a task? fNIRS can add another layer of measurement. It uses light to track changes in blood-related signals. Turning those measurements into useful findings requires a clear match between the question, task and measurement setup.

Introduction

How can you investigate what happens when someone follows instructions, uses an interface or learns a task? fNIRS can add another layer of measurement. It uses light to track changes in blood-related signals. Turning those measurements into useful findings requires a clear match between the question, task and measurement setup.

Summary

fNIRS stands for functional near-infrared spectroscopy. It investigates brain activity indirectly through changes in oxygenated and deoxygenated haemoglobin. Measurements are primarily sensitive to superficial cortex beneath the measurement locations. A signal difference alone is not a score for preference, understanding or purchase intention. Scholkmann et al., 2014

Where does fNIRS come from?

In 1977, Frans Jöbsis published work on non-invasive infrared monitoring of oxygen-related processes, including in the brain. This provides a historical foundation for NIRS. It does not validate present-day marketing applications. Jöbsis, 1977

The addition of functional refers to investigating function. A research brief should therefore explain which task or comparison will be used and what conclusion the findings are intended to support.

How does the light measurement work?

Light sources and detectors placed on the head are called optodes. Oxygenated haemoglobin (HbO) and deoxygenated haemoglobin (HbR) absorb light differently. Common continuous-wave systems estimate relative concentration changes from intensity changes using the modified Beer–Lambert law. They do not measure electrical brain activity or directly provide percentage oxygen saturation. Scholkmann et al., 2014

When examining a graph, first ask what its lines represent. Are they HbO, HbR, differences between conditions or a derived index? What units and reference are used? Without this information, a colour or peak is difficult to assess.

What else can influence the signal?

Scalp blood flow and changes in systemic physiology can affect the recorded signal. An effect may consequently be attributed to brain activity incorrectly or go undetected. Tachtsidis and Scholkmann, 2016

Short-separation channels are predominantly sensitive to superficial tissue. Researchers can use them to estimate and reduce contributions from outside the brain. Contact quality, movement and excluded data also need reporting. Such correction does not guarantee a completely isolated brain signal. Yücel et al., 2021

Turn this into a practical question: how does the study establish that its preferred explanation is more plausible than a change in movement, physical effort or sensor contact?

Can fNIRS be used outside the laboratory?

Wearable setups are available. A systematic review of postural and walking research illustrates the potential of fNIRS for movement science, while identifying methodological differences that make studies difficult to compare. Herold et al., 2017

For an applied study, start with a pilot in which participants perform the actual task. Assess whether the setup suits those activities. A successful seated demonstration does not establish feasibility during bending, speaking and walking. Agree in advance when insufficient usable data should trigger a change in task or method.

Which research questions might fit?

These are briefing examples, not validated applications of a particular device.

Proposed questionFirst clarify
Which instructions better support the task?Which performance measure matters, and what does the physiological measurement add?
What changes during learning?How will practice be distinguished from differences in difficulty?
How does a task unfold in practice?Which events, movements and conditions will be recorded?
Which communication works better?Which behaviour should change, and how will it be tested separately?

Do not substitute a physiological outcome for the actual research objective. If the aim is fewer errors, errors also need to be measured.

Assess a proposal in six steps

  1. Name the decision. For example, choosing between two instructions for the same action.
  2. Define the comparison. Specify what stays constant and what deliberately changes.
  3. Select the outcomes. Identify the performance, experience or choice that matters alongside fNIRS.
  4. Request a justified setup. Ask which regions are sampled and which quality criteria apply.
  5. Specify the analysis beforehand. Explain how exclusions, confounds and uncertainty will be handled.
  6. Agree on the next step. Decide when the findings warrant a revision, another test or no change.

This is an editorial briefing aid. The actual design and sample require their own scientific justification.

Example: comparing two work instructions

Imagine an organisation comparing written instructions with instructions using illustrations. Both explain the same action at a workstation. This example is fictional; it contains no measurements or client findings.

ElementSuggested briefing specification
DecisionWhich instructions proceed to a practical trial?
TaskThe same action, materials and available time
SequenceA design accounting for learning and order effects
BehaviourErrors, completion and assistance required
ExperienceBrief questions about clarity and perceived effort
fNIRSA predefined comparison with documented quality criteria

Agree how conflicting outcomes will be discussed. Instructions could produce fewer errors while a physiological measure changes. This alone does not justify calling them “easier”. The conclusion should reflect all relevant evidence and its uncertainty.

Common interpretation mistakes

  • Treating a higher line as better. Ask which hypothesis supports that interpretation.
  • Using a figure without a reference. Show the task, comparison, units and variability.
  • Leaving exclusions invisible. Ask how much data was usable and whether exclusions could bias the comparison.
  • Generalising from one task to all customers. State which participants and circumstances the conclusion covers.
  • Inferring an associative target group from one signal. In this knowledge base, this means a bounded group making decisions on the basis of shared associations. That working definition requires research into associations and decisions; an fNIRS pattern alone does not establish the grouping.

What should you take away?

Assess fNIRS through the question you need to answer. Ask what it adds, how alternative explanations will be examined and which decision the findings can support. Also agree on consent, access to data and the use of conclusions. This article explains a method; it is not a diagnostic protocol or a tool for evaluating individual employees.

Key terms

fNIRS
fNIRS stands for functional near-infrared spectroscopy. It investigates brain activity indirectly through changes in oxygenated and deoxygenated haemoglobin. Measurements are primarily sensitive to superficial cortex beneath the measurement locations. A signal difference alone is not a score for preference, understanding or purchase intention. Scholkmann et al., 2014
HbO and HbR
Light sources and detectors placed on the head are called optodes. Oxygenated haemoglobin (HbO) and deoxygenated haemoglobin (HbR) absorb light differently. Common continuous-wave systems estimate relative concentration changes from intensity changes using the modified Beer–Lambert law. They do not measure electrical brain activity or directly provide percentage oxygen saturation. Scholkmann et al., 2014
Short-separation channels
Scalp blood flow and changes in systemic physiology can affect the recorded signal. An effect may consequently be attributed to brain activity incorrectly or go undetected. Tachtsidis and Scholkmann, 2016

Frequently asked questions

What does fNIRS stand for?

fNIRS stands for functional near-infrared spectroscopy.

What do HbO and HbR mean?

HbO refers to oxygenated haemoglobin; HbR refers to deoxygenated haemoglobin. Ask which quantity and reference a report presents.

Can fNIRS be used outside the laboratory?

Wearable setups exist. Use a pilot to establish whether the setup and data quality suit the actual task.

Does a higher signal mean something works better?

That conclusion requires justification. Define the interpretation to be tested beforehand and combine the result with relevant behavioural and experience measures.

How many participants are needed?

Request a justification based on the question, outcome, required precision and expected exclusions. This article does not provide a universal minimum.

Does fNIRS replace a usability test?

Not automatically. Ask which additional uncertainty it should reduce. If behaviour or interviews can answer the question sufficiently, the extra measurement needs a separate justification.

Sources

  1. 1.F. F. Jöbsis (1977). Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science 198(4323), 1264–1267. - Science (1977)
  2. 2.F. Scholkmann, S. Kleiser, A. J. Metz et al. (2014). A review on continuous wave functional near-infrared spectroscopy and imaging instrumentation and methodology. NeuroImage 85(Pt 1), 6–27. - NeuroImage (2014)
  3. 3.M. A. Yücel, A. v. Lühmann, F. Scholkmann et al. (2021). Best practices for fNIRS publications. Neurophotonics 8(1), 012101. - Neurophotonics (2021)
  4. 4.I. Tachtsidis, F. Scholkmann (2016). False positives and false negatives in functional near-infrared spectroscopy: issues, challenges, and the way forward. Neurophotonics 3(3), 031405. - Neurophotonics (2016)
  5. 5.F. Herold, P. Wiegel, F. Scholkmann, A. Thiers, D. Hamacher, L. Schega (2017). Functional near-infrared spectroscopy in movement science: A systematic review on cortical activity in postural and walking tasks. Neurophotonics 4(4), 041403. - Neurophotonics (2017)

Related topics

Reviewed by: Martijn den Otter · Last reviewed: 9/29/2026

Martijn den Otter

Martijn den Otter

Oprichter van Neurofactor. Expert in neuromarketing en consumentenpsychologie.

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